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Subject: Ligand lanes genet. The ligand lanes of a ProteOn GLC Sensor Chip were activated with EDC/NHS (flow rate 30µl/min) and HSA was injected at 10µg/ml in ProteOn Acetate buffer pH4.5 (flow rate 100µl/min) to render immobilization levels of approximately 3200 RU. After immobilization, surfaces were deactivated with ethanolamine HCl (flow rate 30µl/min). Nanobodies were injected for 2 minutes at 45µl/min over the HSA surface to render a Nanobody capture level of approximately 200 RU.Как в данном случае переводится ligand lanes? Lanes - это дорожки? |
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