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 dao2812

link 8.08.2016 9:37 
Subject: Shotgun Mutagenesis Epitope Mapping biol.
Помогите, пожалуйста, перевести название раздела:

"Shotgun Mutagenesis Epitope Mapping"

Последующий контекст:
Various amino acid residue of interest for anti-MCAM antibody binding were identified using shotgun mutagenesis and high-throughput cellular expression technology that enables the expression and analysis of large libraries of mutated target proteins within eukaryotic cells.

Every residue in the human MCAM protein was individually mutated to an alanine, or other specified residue, to assay changes in function.

Proteins were expressed within standard mammalian cell lines.

Спасибо!

 alk

link 8.08.2016 11:22 

 dao2812

link 8.08.2016 12:43 
спасибо! картирование эпитопов с помощью мутагенеза методом дробовика?

 

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