Subject: dropout media gen. Transformed strains were grown in the appropriate complete minimal dropout media, supplemented to 100 mg/L adenine hemisulfate.To monitor growth on cello-oligosaccharides, engineered strains were grown in 5 mL of complete minimal media with appropriate dropouts overnight. These starter cultures were washed three times with 25 mL of ddH20, and resuspended to an OD (at 600 nm) of 0.1 in Yeast Nitrogen Base (YNB) plus the appropriate Complete Supplemental Media (CSM) |
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