|
Subject: occasional swirling chromat. occasional swirlingPlacebo Preparation: Weigh placebo powder accurately equivalent to about 10 intact tablets into 100ml volumetr
flask. Add 10 ml of water and sonicate with occasional swirling to disperse the table
completely. Add about 70 ml of diluent and sonicate for 45 minutes in cool water. Allow th
solution to attain the room temperature and dilute up to the mark with diluent and mi
Centrifuge the content at 3500 RPM for 10 minutes in stoppered centrifuge tube. Dilute 5 ml
this supernatant solution to 50 ml with diluent and mix. Filter the solution through 0.45µ nylo
filter discarding first 2 ml of filtrate. thank you
|