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Subject: 550 ng of Nco/Not cut insert biol. 550 ng of Nco/Not cut insert was also Iigated into the Ncol and Not 1 sites of pD6 (2.4 pg) to create a construct expressing a fusion between the scFv and the Fc region of human lgG2. Ligated DNA was transformed into electro-competent NEB5alpha cells (New England Biolabs, Cat C2989) which generated a library size of 2-3 x107 clones for each population.помогите, пожалуйста, перевести "550 ng of Nco/Not cut insert " Заранее большое спасибо! |
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включили фрагмент (insert DNA) в плазмиду pD6 для этого было взято 550 нг of insert DNA предварительно фрагмент был "вырезан" с одного конца по участку Nco, а с другого - Not. |
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