Subject: carbohydrate-oligosaccharide mapping; FACS-based single-cell cloning biol. Помогите, пожалуйста, улучшить перевод. Очень много непонятных терминов: carbohydrate-oligosaccharide mapping; FACS-based single-cell cloning; Antibodies produced by single cell clones can also be tested for turbidity:Having introduced vector(s) encoding antibody heavy and light chains into cell culture, cell pools can be screened for growth productivity and product quality in serum-free media. Top-producing cell pools can then be subjected to FACS-based single-cell cloning to generate monoclonal lines. Specific productivities above 50 pg or 100 pg per cell per day, which correspond to product titers of greater than 7.5 g/L culture, can be advantageous. Antibodies produced by single cell clones can also be tested for turbidity, filtration properties, PAGE, IEF, UV scan, HP–SEC, carbohydrate-oligosaccharide mapping, mass spectrometry, and binding assay, such as ELISA or Biacore. A selected clone can then be banked in multiple vials and stored frozen for subsequent use. Спасибо!!! |
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