Subject: "in-frame cloning into exogenous peptides" and "inserted in-frame" biol. The plasmid containing the nucleotide sequence encoding Invasin (pHIT123-Inv) was used as a PCR template, and oligonucleotide primers were designed to amplify the minimal sequence encoding the beta-1 integrin binding site (-600 bp) while introducing restriction sites for in-frame cloning into exogenous peptides for targeted delivery."The resulting product is then inserted in-frame" with exogenous peptides such as PBK10 for targeting such delivery proteins to cells expressing CD4 (i.e. "helper" T-cells). Помогите, пожалуйста, пересети "in-frame cloning into exogenous peptides" and "inserted in-frame" клонирование в рамку считывания .....экзогенных пептидов? Спасибо! |
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