Acceptor FRAMEWORK FOR CDR GRAFTING biol.

[dao2812]

Acceptor FRAMEWORK FOR CDR GRAFTING

Humanized antibodies were also engineered, in which the rodent sequence of the variable region itself is engineered to be as close to a human sequence as possible while preserving at least the original CDRs, or where the CDRs from the rodent antibody were "grafted into framework" of a human antibody (e.g., Riechmann, Nature 1988 332:323-7; US5,693,761).

Hence, the grafting of non-human CDRs antibodies into a human framework is a major protein engineering task.
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Often antigen binding affinity is greatly reduced or abolished after loop grafting.
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The use of carefully selected human frameworks in grafting the antigen binding loops maximizes the probability of retaining binding affinity in the humanized molecule (Roguzka et al 1996)

Как перевести GRAFTING в данном случае?

Спасибо!