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Subject: cells expressing the consensus clade A Env plasmid biol. However, for both the purified VRC01 mAb and sera from pHIV-1Env-Fab administered mice there was significant positive staining of transfected "cells expressing either the consensus clade A Env plasmid" (pCon-Env-A) as well as an "optimized clade C plasmid" (pOpt-Env-A) expressing and Env from the primary HIV-1 isolate pQ23Env17."DNA expressing either a consensus (pCon-Env-A) or “optimized” (pOpt-Env-A) HIV-1 clade A envelope" was transfected into 293T cells. ELISA immunoassay, using a consensus HIV-1 clade B gp120 protein, confirmed the presence of the anti-HIV-1 Env-Fab in the supernatant from the transfected 293 T cells as early as 24 hours post transfection (FIG. 5C). Помогите, пожалуйста, разобраться. |
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link 28.04.2015 0:27 |
| В чем именно? |
| в этом построении "cells expressing the consensus clade A Env plasmid" |
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An ELISA immunoassay, using a "consensus HIV-1 clade B gp120 protein", confirmed the presence of the anti-HIV-1 Env-Fab in the supernatant from the transfected 293 T cells as early as 24 hours post transfection (FIG. 5C). К чему относится consensus ? |
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