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Subject: " very low lower limits of quantification" biol. Coefficients of variation (CV’s) in buffer signals with perchlorate elution were measured over 8 replicates, reasoning that signals close to machine background were entitled to be noisy, and thus could preclude realization of the very low lower limits of quantification (LLOQ) suggested by these very low backgrounds. In this experiment, with a median signal of just 32 RFU, the raw median CV was 6.2% (Figure 6). Stability at these very low signals was not likely to be a factor that limits LLOQ.Спасибо!
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