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Subject: assembly of plated were spun biol. Photocleavage
The filter plates were blotted on absorbent pads and were irradiated for 6 min with a BlackRay UV lamp with shaking (800 rpm, 25°C). The plates were rotated 180 degrees and irradiated for an additional 6 min under the BlackRay light source. The 40% filter plate was placed onto an empty 96-well plate. The 1% filter plate was stacked on top of the 40% filter plate and the 0.005% filter plate was stacked on top of the 1% filter plate. ""The assembly of plated were spun"" for 1 min at 1000x g. The 96-well plate with eluted sample was placed onto the robot deck. 60% glycerol in 1xSB17,Tw from the 37°C incubator was placed onto the robotic deck.
Спасибо!
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