Subject: Cell Maintenance Procedure immunol. Уважаемые коллеги, как лучше перевести "Cell Maintenance Procedure" - процедура обслуживания клеток, процедура поддержки клеток, процедура культивирования клеток иои как-то иначе?Контекст следующий: 1. Add approximately 50.0 mL of cell maintenance medium to 175 cm2 tissue culture fiask(s). 2. Decant conditioned medium from a confluent culture of MDBK cells. 3. Add 5 mL of trypsin solution to the 175 cm2 flask, replace cap and place into 37°C incubator until cells are loosened from surface of the flask; incubate 5 to 10 minutes. 4. For a 175 cm2 flask, break up clumps of cells by repetitive drawing the cell suspension into a 10-mL pipette and expelling it, then add 5 mL of cell maintenance medium to the cell suspension. 5. Incubate flask in a 37°C incubator having a 5% CO2 atmosphere until cultures grow to a nearly confluent monolayer of cells. Заранее спасибо. |
По- моему, всё равно - "питательная среда" |
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