DictionaryForumContacts

 Фарма

link 20.08.2011 16:33 
Subject: Cell Maintenance Procedure immunol.
Уважаемые коллеги, как лучше перевести "Cell Maintenance Procedure" - процедура обслуживания клеток, процедура поддержки клеток, процедура культивирования клеток иои как-то иначе?
Контекст следующий:
1. Add approximately 50.0 mL of cell maintenance medium to 175 cm2 tissue culture fiask(s).
2. Decant conditioned medium from a confluent culture of MDBK cells.
3. Add 5 mL of trypsin solution to the 175 cm2 flask, replace cap and place into 37°C incubator until cells are loosened from surface of the flask; incubate 5 to 10 minutes.
4. For a 175 cm2 flask, break up clumps of cells by repetitive drawing the cell suspension into a 10-mL pipette and expelling it, then add 5 mL of cell maintenance medium to the cell suspension.
5. Incubate flask in a 37°C incubator having a 5% CO2 atmosphere until cultures grow to a nearly confluent monolayer of cells.

Заранее спасибо.

 c_khrytch

link 20.08.2011 20:18 
По- моему, всё равно - "питательная среда"

 

You need to be logged in to post in the forum

Get short URL | Photo