Subject: four parameter logistic fit pharm. Помогите, пожалуйста, разобраться с "four parameter logistic fit" в следующем контексте:Briefly, ELISA plates were coated with goat anti-biotin antibody (5 μg/ml, 4° C., overnight), blocked with Superblock (Pierce), and incubated with biotinylated human IL-17 (IL-17 capture ELISA) or IL-20 (IL-20 capture ELISA) at 50 ng/ml in 10% Superblock TTBS at room temperature for 2 h. Serum samples were serially diluted (0.5% serum, 10% Superblock in TTBS) and incubated on the plate for 30 min at room temperature. Detection was carried out with HRP-labeled goat anti human antibody and concentrations were determined with the help of standard curves using the four parameter logistic fit. Спасибо! |
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link 6.06.2016 16:02 |
4-параметрическая логарифмическая логистическая модель (4PL) |
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