Subject: automated fluorescence microscopy scanning system biol. The chip with CTC was incubated with primary antibodies against wide-spectrum cytokeratins (Abcam), CD45 (Santa Cruz Biotechnology) and Ki67 (Life Technologies) and secondary antibodies conjugated with Alexa Fluor 647, Alexa Fluor 555 and Alexa Fluor 488 (all from Life Technologies).An automated fluorescence microscopy scanning system (BioView) was used to identify Ki67-positive CTCs (CK/CD45//Ki67), Ki67-negative CTCs (CK/CD45/Ki67) and contaminating white blood cells (CD45). Как перевести automated fluorescence microscopy scanning system?
|