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Subject: multiplexing capacity biol. For multiplexed detection of a small number of aptamers still bound to the second solid support, fluorescent dyes with different excitation/emission spectra can be employed to detect and quantify two, or three, or five, or up to ten individual aptamers.These mass labels can also incorporate different fluorescent or chemiluminescent dyes for additional """"multiplexing capacity"""". Labels may be added indirectly to aptamers by using a specific tag incorporated into the aptamer, either during synthesis or post synthetically, and then adding a probe that associates with the tag and carries the label. Спасибо! |
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